An efficient protocol for regeneration in Indian mustard

Brassica  juncea (L.) Czern and Coss

SEEMA K. SINGH, D.P. MISHRA , PANKAJ K.  JOSHI and SANDEEP ARORA¹

Department of Biochemistry,

¹Department of Molecular Biology and Genetic Engineering,

College of Basic Sciences and Humanities,

G.B. Pant University of Agriculture and Technology,

Pantnagar-263 145 (U.S. Nagar, Uttarakhand)

ABSTRACT

 An efficient protocol for plant regeneration from hypocotyl explants has been developed in two Brassica juncea cultivars, namely Pusa Jai Kisan and Varuna. The two cultivars are among the top ten cultivars used in India. Different combinations of NAA and BAP were used to obtain optimal regeneration response. The cultivars showed a varied shoot regeneration response. Regeneration frequency was slightly higher in the Pusa Jai Kisan (85.71%) than Varuna (83.3%). Ninety per cent of the regenerated plantlets showed rooting response and 65 per cent of the rooted plantlets were successfully transferred to pots.