A study of diversity in germplasm collection of hexaploid wheat by RAPD

analysis

SANJEEV KUMAR¹ and V. K. KHANNA²

¹ Department of Molecular Biology and Genetic Engineering,

College of Basic Sciences and Humanities

² Department of Genetics and Plant Breeding,

College of Agriculture,

G. B. Pant University of Agriculture and  Technology,

 Pantnagar-263145 (U. S. Nagar, Uttarakhand)

ABSTRACT

 Polymerase chain reaction (PCR) based randomly amplified polymorphic DNA (RAPD) markers were used to study the genetic relationship and genetic diversity among 15 Indian wheat accessions. Screening was done with 6 random primers. All of the 32 amplified RAPD bands were polymorphic. The similarity coefficient between hexaploids ranged from 0.00 to 0.80. Primers UBC 552, UBC 18 and UBC 535 showed maximum number of banding pattern and were able to distinguish eight varieties. Primer UBC 572, UBC 18, UBC 337, and UBC 535 were found to give a distinguishable number of unique RAPD markers. Dendrogram showed that variety HD 2687 was very diverse.